Mapping Structural Changes of Eukaryotic Elongation Factor-2 Kinase using 351 nm UVPD-MS

摘要

351-nm UVPD paired with chromogenic reagents allow a facile and confident identification of NN-modified peptides. This UVPD-based methodology allowed the elucidation of the most reactive lysine and cysteine residues, ones which are anticipated to reflect high surface exposure. The NN chemical probes can also be used to elucidate calcium/calmodulin induced structural changes. For proteins without crystal or NMR structures, chemical probe methods offer the first step towards building conformational maps of these larger proteins.