An Improved Immunosuppressant Drug Research Method Based on a Novel SPLC-MS/MS System

摘要

Improved reliability and economy was achieved for ISD analysis for research purposes by using a novel SPLC-MS/MS system and method. 1. Ion suppression of ISDs by co-eluting phospholipids was largely avoided by using the short Accucore C8 HPLC column. 2. Using 1/x weighting, correlation coefficients (r2) > 0.995 were typical for: (1) Cyclosporin A, from 25 to 1250 ng/mL, (2) Everolimus, Sirolimus & Tacrolimus, from 2.5 to 50 ng/mL. 3. Carryover, measured by peak areas corresponding to the ISDs from blank injections following the highest calibrators, was typically less than 0.1%. 4. Reproducible ISD QC results were obtained from three research test sites evaluating this method with the Prelude SPLC-TSQ Vantage system. 5. A reduction in solvent waste of about 40% was achieved, comparable to legacy TurboFlow methods for ISDs.