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A Robust LC-MS based Workflow for Comparative Glycoproteomics with Online Glycopeptide Enrichment and Separation

机译:基于鲁棒的LC-MS工作流程,用于对比较糖蛋白质具有在线糖肽富集和分离的

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摘要

The HILIC-C18 platform greatly reduces sample complexity for bottom-up glycopeptidomics. This is a novel method for robust online enrichment and LC-MS analysis of glycopeptides, without any bias involving sialylated or non-sialylated species. This platform will serve at the center of a multidimensional glycoprotein analysis workflow, to provide a rapid and comprehensive analysis of both the peptide and sugar moieties.
机译:HILIC-C18平台大大降低了对自下而上糖肽的样本复杂性。这是一种新的糖尿病在线富集和LC-MS分析的新方法,没有任何涉及唾液酸化或非唾液酸化物种的偏差。该平台将在多维糖蛋白分析工作流程的中心服务,以提供对肽和糖部分的快速和综合分析。

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