Novel Method for Extracting Proteins from Unused Phases after Nucleic Acid Extractions from TRIzol for Mass Spectrometry-base d Proteomics

机译：从核酸萃取后从核酸萃取后萃取蛋白质从核酸萃取蛋白质碱基碱基D蛋白质组学的新方法

获取原文

页面导航

摘要
著录项
相似文献
相关主题

摘要

FASP-buffer successfully reconstituted 91% of the TRIzol-extracted protein, yielding the highest reported protein yields of 100 μg/ mg of mosquito tissue. The improved TRIzol-extraction-re-solubilization method was implemented to isolate proteins after nucleic acid extractions to generate transcriptomic and proteomic data from a single sample.

机译：FASP缓冲液成功地重构了91％的三唑提取的蛋白质，产生了最高报告的蛋白质产量为100μg/ mg蚊组织。实施了改进的三唑 - 提取重溶解方法以在核酸萃取后分离蛋白质以产生来自单个样品的转录组和蛋白质组学数据。

著录项

来源
《American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics》|2012年||共1页
会议地点
作者
Arti T. Navare; David E. Purdy; Angela L. Rasmussen; Victoria S. Carter; Lisa Jones; Phil Gafken; Yan-Jang S. Huang; Dana L. Vanlandingham; Stephen Higgs; Alexei L. Krasnoselsky; Michael G. Katze;
展开▼
作者单位

展开▼
会议组织
原文格式 PDF
正文语种
中图分类 TB994.5-53;
关键词

相似文献

外文文献
中文文献
专利

1. Extraction of Nucleic Acids Comparison of automated and manual Extraction Methods from nucleic acid-based Detection Systems in the Food Laboratory [J] . Lorenzen Erika Deutsche Lebensmittel-Rundschau . 2020,第7期

机译：核酸核酸与食品实验室中核酸的检测系统自动化和手动提取方法的比较
2. Efficient recovery of proteins from multiple source samples after trizol ? or trizol ? LS RNA extraction and long-term storage [J] . André ES Sim?es, Diane M Pereira, Joana D Amaral, BMC Genomics . 2013,第1期

机译：Trizol后可从多种来源的样品中有效回收蛋白质？还是三唑？ LS RNA提取和长期保存
3. Influenza and respiratory syncytial virus detection in clinical specimens without nucleic acid extraction using FOCUS direct disc assay is substantially equivalent to the traditional methods and the FOCUS nucleic acid extraction-dependent RT-PCR assay [J] . SelvarajuS.B., TierneyD., LeberA.L., Diagnostic microbiology and infectious disease . 2014,第3期

机译：不使用FOCUS直接圆盘测定法提取核酸的临床标本中的流感和呼吸道合胞病毒检测基本上等同于传统方法以及FOCUS依赖核酸提取的RT-PCR测定法
4. Novel Method for Extracting Proteins from Unused Phases after Nucleic Acid Extractions from TRIzol for Mass Spectrometry-base d Proteomics [C] . Arti T. Navare, David E. Purdy, Angela L. Rasmussen, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2012

机译：从核酸萃取后从核酸萃取后萃取蛋白质从核酸萃取蛋白质碱基碱基D蛋白质组学的新方法
5. Mass spectrometry dissociation studies of protein-protein and protein-nucleic acid complexes and 13C flux of amino acids. [D] . Ma, Xin. 2014

机译：蛋白质-蛋白质和蛋白质-核酸复合物的质谱解离研究以及氨基酸的13C通量。
6. Evaluation of the Use of TRIzol-Based Protein Extraction Approach for Gel-Based Proteomic Analysis of Dried Seafood Products and Chinese Tonic Foods [O] . Kin-Ka Chan, Celia Sze-Nga Kwok, Eric Tung-Po Sze, 2018

机译：基于TRIzol的蛋白质提取方法在基于凝胶的蛋白质组学分析中对干海鲜产品和中药食品的评价
7. Extraction of total nucleic acids from bacterial isolates using the bioMérieux NucliSENS easyMAG total nucleic acid extractor [O] . Eleanor A. Powell, Joel E. Mortensen 2016

机译：使用bioMérieuxNucliSENS easyMAG总核酸提取器从细菌分离物中提取总核酸
8. Suvivin-Binding Proteins, Encoding Nucleic Acids, and Methods of Use (PAT APPL-11-396 009). [R] . Reed, J. C., Okada, K. 2006

机译：suvivin结合蛋白，编码核酸和使用方法（paT appL-11-396 009）。

Novel Method for Extracting Proteins from Unused Phases after Nucleic Acid Extractions from TRIzol for Mass Spectrometry-base d Proteomics

摘要

著录项

相似文献

相关主题

期刊订阅