Simple LC/MS/MS Screening and Quantify Methods for the Analysis of 41 Common Pain Drugs in an Oral Fluid Matrix For Research Use Only. Not for Use in Diagnostic Procedures.

摘要

The detection of several compound classes, such as common pain panel and drugs of abuse is of much importance. While this analysis has generally been utilized for urine sample matrices, many research laboratories are moving forward to analyze these compounds in the oral fluid matrices. The basis for this testing needs to provide a means to accurately and precisely monitor these compounds in a timely fashion, while having a simplified workflow that is conducive to their setting. This current effort has shown to accomplish just that. In this study we present a fast, robust, and reliable method, that has afforded the detection of 41 compounds in an oral fluid matrix. The LC/MS/MS method was achieved using the Scheduled Multiple Reaction Monitoring (Scheduled MRM algorithm) detecting all compounds with Limits of Quantitation (LOQ) inline with the current recommended cutoffs. In addition to the above, the MPX -2 high throughput HPLC system was utilized to provide an increase in sample throughput of 171%. In addition to the above, the screening method was able to achieve both quantitation and spectra confirmation which can be used for searching against the library of all 41 pain drugs in one injection was also achieved in a short run due to the fast scanning speed in the QTRAP 4500 system.