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METHOD FOR QUANTIFICATION OF PEPTIDE AND PROTEIN BY LC/MS/MS
METHOD FOR QUANTIFICATION OF PEPTIDE AND PROTEIN BY LC/MS/MS
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机译:LC / MS / MS定量肽和蛋白质的方法
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摘要
Disclosed is a method which can quantify a specific protein or peptide contained in a trace amount with high accuracy and in a simple manner without the need of using any expensive reagent. A protein or peptide of interest can be quantified by LC/MS/MS by using, as an internal standard, a protein or peptide comprising an amino acid sequence having the reshuffling the binding order of amino acid residues in the amino acid sequence for the protein or peptide of interest.
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机译:公开了一种无需使用任何昂贵的试剂就可以以简单且准确的方式对痕量的特定蛋白质或肽进行定量的方法。感兴趣的蛋白质或肽可以通过LC / MS / MS进行定量,方法是使用包含氨基酸序列的蛋白质或肽作为内标,该氨基酸序列具有重新排列该蛋白质氨基酸序列中氨基酸残基的结合顺序或感兴趣的肽。
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