Identification of Hypoxia-Induced Metabolic Changes in Human Intestinal Epithelial Cells

摘要

A high resolution Q- TOF utilizing MS~E was utilized to prof ile differences in hypoxia- induced metabolites in T84 intestinal epithelial cells. Over 2,000 features (m/z, abundance and retention time) were identified in each sample. Potential markers were selected using multivariate Splots; structural candidates were identified by searching an internal database, as well as interrogation of Metlin and ChemSpider. Interesting candidates validated by MassFragment included UDP - N - acetyl - D - galactosamine and its structural isomers. UDP- N-acetyl-D-galactosamine (UDP- GalNA c) is the amino sugar nucleotide donor of N- acetyl - D - galactosamine (GalNAc) residues and plays a role in the O - glycosylation of proteins. Hypoxia- induced protein glycosylation represents a novel response to oxidative stress in T84 cells. Future efforts w ill include the use of chromatography, ion mobility and NMR to determine which of these structural isomers is upregulated in hypoxia.