High throughput LC/MS/MS analysis of serotonin re-uptake inhibitors from protein precipitated plasma samples.

摘要

Filtration using a non-drip filtration plate provides near complete protein removal compared to that of centrifugation. Protein levels in the post plate eluent were low enough to be undetected. The assay was improved using a non-drip filtration plate. RSDs were reduced by 4percent over the protein precipitation procedure. Response as measured by peak area was improved for most compounds. High throughput analysis of human samples is slow and difficult with standard centrifugation. The use of filtration plates facilitates the high throughput automation of protein precipitation required in the analysis of SSRIs from human samples, and is suitable for a number of analyses. A new non-drip filtration plate design allows for the automation of protein precipitation without the mess associated with standard filtration plates.