Inter-species Comparison of Metabolite Profiles for Propranolol in Rat and Human Liver Microsomes

摘要

1. Propranolol was observed as the substrate for P450-catalyzed hydroxylation and dealkylation in both rat and human liver microsomes. 2. Among the identified mono-oxidated metabolites, two mono-oxidated metabolites were detected only in human liver microsomes, while the other two were detected only in rat liver microsomes. All these four mono-oxidated metabolites were structurally related isomers and eluted at different retention times. 3. The locations of the mono-oxidation on propranolol in rat and human liver microsomes were putatively proposed based on the obtained MS~(2) and MS~(3) data.