High speed data acquisition and polarity switching MS/MS applied to water-soluble vitamin analysis using a novel multi-mode ODS separation

摘要

Vitamins are essential for healthy growth and development and the need to analyze multivitamin products in the food and pharmaceutical industries is becoming increasingly important. However, the analysis of water-soluble vitamins (B and C) is compromised by the polar nature and poor retention characteristics. A mixed mode bonded silica chemistry using ODS+cation+anion ligands has the advantage that it can be used to separate highly polar compounds and exhibits similar behavior to ODS columns for non-ionic compounds whilst the IEX ligands provide a normal phase interaction for separation. This column chemistry was applied to the simultaneous analysis of water-soluble vitamins using fast polarity switching and high speed data acquisition MS/MS analysis.