Global Metabolomics of Colon Cancer by Analysis of Human Plasma with LC/(+)ESI-MS

摘要

Global metabolomics was applied to plasma from colon cancer patients and healthy controls with LC/MS analysis and statistical software. Statistics performed included PCA, Student's t-tests, PLS-DA and FC analyses. The 16 most intense FCs were examined (Figure 6) and putative chemical formulas/compound names determined (Table 1) with the METLIN database. The PLS-DA loadings examined 13 significant features in detail (Figure 10) and METLIN was used to determine putative chemical formulas/compound names (Table 2). Those cancer patients observed to group with controls in PCA and PLS-DA plots were missing many of the potential biomarkers shown in Figure 6. Although no known pharmaceuticals were administered to patients, METLIN assignments are being investigated in order to remove potential compounds due to alternative treatments from the statistical analysis. Future work will include structural elucidation and identification of significant features by MS/MS and NMR techniques, as well as determining the biological significance of the putative biomarkers.