Pre-equilibrium In vivo Soild Phase Microextraction for Monitoring Drug Concentration Changes in Brain Tissue

摘要

An advantage of pre-equilibrium solid phase microextraction (SPME) method has been utilized to quantitatively determine the concentrations of carbamazepine from the cortex and striatum regions of the brain of freely moving rats. The method was validated with a parallel microdialysis sampling and the results compared with conventional plasma sample analysis. A 10-min extraction was employed for SPME extractions while 30 min sampling time was required for MD. A pharmacokinetic studies of carbamazepine in both steady and dynamic states was investigated using microdialysis and SPME. Comparable results were obtained for both methods. Drug concentrations in the cortex and striatum measured with solid phase extraction method did not show any significant differences. The CV(percent) recorded for both methods were <= 15.