Using quantitative LC-MS/MS for comparative analysis of monoclonal antibodies for cardiac troponin I

摘要

An LC-MS/MS immunoassay platform can quantitatively select optimal mAbs to be used as capture antibodies for development of antibody-based measurement procedures. Metrological rigor associated with ID MS provides a tool for objective selection of mAbs in analytical applications. Based on data of relative K_(d) constants, we have selected clone 19C7 as a candidate capture mAb for development of cTnI immunoassay measurement procedures. A well-characterized reference measurement procedure based on mass spectrometry can be a valuable complementary tool for immunoassay development & mAb characterization.