Thymic stromal lymphopoietin (TSLP) is an IL-7 like cytokine that was originally identified from the conditioned medium of the Z210R.1 thymic stromal cell line. TSLP requires a heterodimeric receptor complex, IL-7 receptor alpha subunit and its unique TSLP receptor (TSLPR, also known CRLF2) to transmit signals into cells. TSLP acts on a number of distinctive cell types and executes various biological functions. Hyperactive TSLP/TSLPR signaling contributes to development of several disorders in humans including asthma and leukemia. However, detailed signal transduction pathways that are responsible for various biological effects of TSLP still remain elusive. Here, we applied the SILAC-based strategy to study the phosphoproteomic changes induced by TSLP as well as induced by leukemic JAK2R683G/TSLPR signaling in cells. Our data shed light on understanding of TSLP signaling pathways and provide potential therapeutic targets for development of novel treatments for TSLP/TSLPR-associated diseases.
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