Accurate; de novo sequencing accuracy reaches 100percent where longest sequences (>100 AA) are assembled. Average sequencing accuracy is 95percent and improves to 97percent when first and last two residues of every sequence are removed. Yields the most accurate MS/MS de novo reconstructions compared to any other automated approach. Robust; high amino acid prediction accuracy is improved from SPS and it is not affected by large numbers of modified peptides. The presence of multiple proteins in the sample does not significantly degrade accuracy. Extensive; generates the longest de novo sequences to date directly from MS/MS analysis without requiring manual annotation or any sequence homology steps. Significant; more can be gained from the protein sample before comparing against a database.
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机译：准确的; DE Novo测序精度达到100％，其中组装了最长序列（> 100AA）。平均测序精度为95％，并且当移除每个序列的第一个和最后两个残留物时，提高到97％。与任何其他自动化方法相比，产生最精确的MS / MS De Novo重建。强壮的;高氨基酸预测精度从SPS改善，并且不受大量改性肽的影响。样品中多种蛋白质的存在不会显着降低精度。广泛的;迄今为止从MS / MS分析中直接生成Longest De Novo序列，而无需手动注释或任何序列同源步骤。重大;在与数据库进行比较之前，可以从蛋白质样品中获得更多。
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