A Fully Automated Symbiosis Online Extraction Method for Determination of Raltegravir in Plasma by LC/MS/MS

摘要

Two robust methods, offline SPE and Symbiosis online SPE were developed and GLP validated for raltegravir with a range of 1.00 ng/mL - 1000 ng/mL. The online method has a short cycle time of 100 seconds with no analytical column needed. HySphere C18HD 7(mu)m cartridge was validated to reuse for five times to further reduce cost. Sample goes through online SPE process before it reaches LC gradient. With the same volume for elution solvent, increasing the injection volume will increase sensitivity but has no negative impact on chromatography. A mixture of TEA and EDTA is the key to eliminate system and SPE cartridge carryover for online method.