Quantitative Determination of Bisphenol A from Human Saliva by Bulk Derivatization and Trap-and-Elute HPLC-ESI-MS

摘要

Use of bulk derivatization in conjunction with restricted access media (RAM) allowed for expedited sample preparation relative to traditional methods while still imparting high recovery levels with enhanced sensitivity. Extensive optimization was conducted on the bulk derivatization reaction as well as the sample preparation protocol in order to reach the optimal conditions outlined in the finalized procedure. Bulk derivatization and RAM also provides a route for the integration of the entire procedure towards a fully automated online quantitative method. Three separate validated methods (a single 5 (mu)L injection method, a multiple injection loading (3 X 20 (mu)L) method, and MIL method with an internal standard) are presented for the detection of trace levels of BPA from human saliva. Two applications of trace BPA analysis from canned food goods are presented demonstrating the viability of these validate methods in a real-world scenario. The detected concentrations of BPA from the canned green chili salsa from Mexico were below the reported limit of detection from method validation. However, the concentrations detected from the hot and sour soup from Taiwan were well within the limit of detection as well as the limit of quantification.