Triacylglycerol Profiling and Positional Isomer Analysis in Omega-3 Enriched Fish Oil by Mass Spectrometry

摘要

Over 60 TAGs were identified in fish oil using neutral-loss triggered product-ion mass spectra and accurate masses of intact TAGs. MS~(3) mass spectra of [M + Li]~(+) adducts of TAGs allowed the sn-positional distribution of fatty acids to be determined. Calibration curves for lithiated adducts were applied to determine isomeric abundance in fish oils. MS~(3) results showed, after re-esterification, higher levels of EPA at position sn-2, and lower levels of DHA at the same position.