Investigation of collision cross section effects on ion motion in ICR cell under the influence of bath gas and quadrupolar excitation

摘要

The level of sample complexity in glycomics is extremely high. Analysis of saccharide variants requires utilization of tandem MS with rapid structure-sensitive separation techniques for their identification. The scheme of analysis in which ion isolation for each stage of the MS~(n) anslysis is performed based on either ion mobility (IM) or m/z or both would be a universal and rapid way of carbohydrate anslysis. The idea of performing IM separation of products at each step of the MS~(n) oligosaccharide analysis was proposed before [1]. In this work we discuss a new implementation of the ion mobility technique which can make this analysis scheme possible. The proposed implementation can also provide means for selective manipulations with mobility-separated protein conformations. It can also allow high-resolution m/z-based precursor ion isolation for MS/MS experiments when an ICR cell is operated at moderate vacuum (10~(-4)-10~(-7) Torr) conditions. We outline the proposed experimental scheme below and present initial results of computer simulations.