Thin-Layer Chromatography (TLC) Coupled with Electrospray Laser Desorption Ionization (ELDI) Mass Spectrometry for High-Throughput Analysis of Mixture

摘要

Electrospray laser desorption ionization mass spectrometry (ELDI/MS) has been demonstrated to be useful in detecting both large and small compounds on the solid under ambient conditions. The analytes were desorbed by using a pulse laser without the application of organic matrix. The desorbed materials were post-ionized in an electrospray plume. The coupling of thin-layer chromatography (TLC) with ELDI/ MS/MS to directly characterize the chemical compounds separated on a TLC plate was demonstrated in our precious study. In this study, we report the design and construction of an automatic TLC-ELDI/MS/MS system for high throughput analysis. The extracts of over-the-counter tablets were separated on the TLC plates. Each of the plate was then characterized by this automatic ELDI system.