Targeted Quantitative Proteomics Using Selected Reaction Monitoring (SRM)-Mass Spectrometry Coupled with an ~(18)O-labeled Reference as Internal Standards

摘要

The utility of 18O-labeled “universal” reference as internal standards for targeted quantitative proteomics has been successfully demonstrated. - A linear dynamic range of quantification approx 10~(4) in relative concentration. - Better reproducibility than label-free approach. - Sufficient effectiveness for monitoring ~(16)O and ~(18)O y-fragments at unit or even higher resolution. Endogenous concentration of CRP and spiked PSA in human plasma successfully determined by “standard protein addition/~(18)O-labeled reference".