We have demonstrated that NETD can interrogate intact proteins. NETD cleaves the protein backbone, producing a- and x-type fragment ions. As the size of a protein increases, ion/ion reactions between protein anions and reagent cations tend to favor hard-sphere collisions over sequence informative backbone cleavages. Future work will examine how pre-, post-, and concurrent energetic activation of the precursor protein anions affects the partitioning between these different product ion populations.
展开▼
