A method for top-down proteomics using Exd and PTR

摘要

The dissociation device cleaves the precursor ions and generates at least two different production ions with overlapping M / Z values within the dissociation device.The dissociation device applies an AC voltage and a DC voltage and generates a pseudopotential that captures ions below the threshold M / Z, including at least two generated ions.The dissociation device accepts charge reducing reagents that charge the captured at least two generated ions until their m / Z values increase above the threshold M / Z set by the AC voltage.The increase in the M / Z values of at least two generated ions reduces their overlapping.At least two generated ions with an increased M / Z value are transmitted to another device for subsequent mass spectrometry by applying a DC voltage to the dissociation device relative to the DC voltage applied to another device.