GC/APCI TOF MS: a new valuable tool for analysis APCI-of biofluids in metabolomics studies

摘要

Derivatization reaction is a crucial step and it is necessary to optimize its conditions in depth. In the positive mode, mostly [M+H]~(+) ions are observed. 25 compounds in the standard mixture were sucessfully detected. Mass accuracies were excellent for the standards with errors of 1ppm and mSigma values at 4.3 in average. The LOD values for the new GC-APCI-TOF-MS method were in the nanomolar range. The GC-APCI-TOF MS method developed is an alternative for the analysis of metabolites in biological fluids.