Thermolytic Digestion of Peptides and Polymers Using Microfabricated Devices with Product Analysis Using DESI and MALDI Introduction

摘要

Thermally generated fragmentation was observed in both MALDI and DESI experiments, and the extent of fragmentation increased as the temperature increased in both oven-heated and resistively-heated experiments. Site-specific cleavage detected using (mu)HP heating matches that previously published using "macro" heating and demonstrates the potential of (mu)HPs to replace bulky glassware. The dramatic changes in fragmentation with relatively small changes in temperature illustrates the need and advantages in using (mu)HPs for thermolytic experiments. The advantage of the precise thermal control allowed by (mu)HPs will enable tuning, optimization, and further improvements and understanding in thermolytic methods.