Development of a Novel Immunoaffinity Capture-Based LC-MS/MS Method for Quantitation of Adrenomedullin in Human Serum

摘要

Developed mass spec based epitope mapping method to locate the region on AM that binds to antibodies, validated by SPR biosensor technology. Developed affinity capture LC-MS/MS method to quantify AM from serum. (1)The nano system achieved a LOQ of 200 amol (6 pg/mL serum). (2)The capillary system achieved a LOQ of 1000 amol (30 pg/mL serum). Developed method to quantify AM bound to CFH. (1)AM concentration is approx3 nM in 0.5 mg/mL commercial CFH samples. (2)Binding stoichiometry approx 1:1000.