Anticancer and antimicrobial properties of peptide fractions from a snow crab byproducts hydrolysate after separation by electrodialysis with ultrafiltration membranes (EDUF)

摘要

Nowadays, the health product market for bioactive peptide obtained from protein hydrolyzates is growing since they demonstrated numerous potential bioactivities. Consequently, it is of great interest to isolate fractions with bioactive properties from complex hydrolyzate [1; 2]. In this context, a new process called electrodialysis with ultrafiltration membranes (EDUF) was developed to perform this separation [3]. Its principle is based on a double separation of peptides according to their electrical charges (electrodialysis) and their molecular masses (ultrafiltration membrane). In this work, two EDUF configurations were used for the hydrolysate separation. In the first one, the electrodialysis cell was composed of 3 different compartments separated by two ultrafiltration membranes with a 20 kDa molecular weight cut-off (MWCO) (figure 1). The first compartment contained the snow crab hydrolysate. The other two compartments contained a solution of a 2g/L KC1 aqueous solution for the recovery of anionic (KC11) and cationic (KC12) peptides respectively. Since this hydrolysate is not characterized, three different pH values were tested to identify the peptide migration way and the peptide quantity recovered for each pH value. The second configuration was composed of nine compartments separated by six ultrafiltration membranes with a 20 kDa MWCO. This second configuration is a triple stacking of the first configuration.