Insoluble carrier to the horseshoe crab blood cell membrane-derived antimicrobial peptides and ligand

摘要

PURPOSE:To obtain the subject insoluble carrier, having a high removal efficiency for pyrogens consisting of bacterial, fungal substances, etc., and useful for purifying pharmaceuticals for injection, etc., by binding an antimicrobial peptide derived from horsecrab corpuscular membranes as a ligand to a carrier, such as agarose. CONSTITUTION:A carrier, such as agarose, is washed and then suspended in water. Sodium hydroxide and epichlorohydrin are added thereto and reaction is carried out while stirring the resultant mixture for 2hr to epoxidize the carrier. An aqueous solution of hexamethylenediamine is subsequently added and reacted therewith to provide an aminohexylated carrier, which is then suspended in a phosphoric acid buffer solution (pH7.0). Glutaraldehyde is subsequently added and reacted therewith. An antimicrobial peptide (e.g. tachyplesin I) derived from horsecrab corpuscular membranes is then added and reacted with the resultant reaction product and sodium borohydride is added to the obtained suspension. Reduction is subsequently carried out to afford the objective insoluble carrier.