ASSAYING OF THE PRESENCE OF H-NS PROTEIN IN A NATIVE STRAIN OF HALOMONAS SPECIES

摘要

H-NS is one of the most abundant nucleoid-associated proteins and has been implicated in the compact organization of the nucleoid structure. It is widely distributed within gram-negative bacteria. H-NS is a small protein that binds DNA in a sequence-independent manner but recognizes curved DNA. Recently, H-NS homologues have been identified in most gram-negative bacteria. Many discovered homologues have low sequence identity with each other (especially at N-terminal domain) and cannot be identified on the basis of sequence homology. The H-NS protein forms oligomers via its N-terminal part and binds DNA fragments via its C-terminal domain, both essential properties of H-NS family proteins . The role of the H-NS proteins in bacterial physiology remains unknown. But it seems to be involved in the regulation of various genes involved in adaptation to environmental challenges.