EVALUATION OF AN ISOTOPE-LABELED MS/MS-CLEAVABLE CROSS-LINKER FOR PROTEIN STRUCTURE ANALYSIS

摘要

Previous studies have shown the benefits of the amine-reactive, CID-MS/MS-cleavable cross-linker DSBU (disuccinimidyl dibutyl urea) for structural proteomics studies based on cross-linking/MS [1, 2]. To further facilitate the automation of cross-linking/MS experiments we now introduce a D12-stable isotope labeled version of the DSBU-linker combining the advantages of MS/MS-cleavable linkers and isotope labeling.