HIGH AFFINITY EPITOPES OF APTAMER COMPLEXES OF THE MULTI-DOMAIN PROTEIN C-MET REVEALED BY PROTEOLYTIC AFFINITY MASS SPECTROMETRY

摘要

C-Met is a glycosylated receptor tyrosine kinase of the hepatocyte growth factor. Upon ligand binding CMet transmits intercellular signals by its unique multi-substrate docking site. However, pathophysiological activation of the C-Met pathway leads to tumorigenesis, schizophrenia and cardiomyocytes death [1]. Here we report a study of the C-Met interaction with two DNA aptamers that bind the target protein (CMet) with high affinity and specificity.