ROBUST AND SENSITIVE LC-MS/MS BASED PLASMA LIPID PROFILING ON A THERMO SCIENTIFIC Q EXACTIVE HF-X MASS SPECTROMETER

摘要

Lipid profiling can be compromised by their extraction from biological samples, chromatographic separation, ionization and detection by mass spectrometry. The Thermo Scientific Q Exactive HF-X is a new Orbitrap based mass spectrometer with increased scan speed and sensitivity, which should both benefit lipid profiling experiments. In this study, we optimized a complete workflow for plasma lipidomics with improved sensitivity and quantitative performances.