PICOLITER PRESSURE-PROBE-ELECTROSPRAY-IONIZATION MASS SPECTROMETRY FOR IN SITU MONITORING SINGLE PLANT CELL METABOLITE CHANGES UNDER STRESS CONDITIONS

摘要

Cytoplasmic streaming occurs consuming the ATP energy formed during glycolysis, respiration and photosynthesis, moving organelles and metabolites all around plant cells. CPP is an instrument that can measure the water status and several properties of intact cells (i.e., volume) [1]. The CPP-mass spectrometer combination (picoPPESI-MS) [2,3] allows to measure water status, cytoplasmic streaming velocities and metabolites in cells sequentially.