THE USE OF PEPTIDE BONDS FOR COLD ION SPECTROSCOPY

摘要

UV absorption by aromatic residues in cold, gas-phase peptides is extremely sensitive to their structure. Combined with vibrational spectroscopy (IR-UV double-resonance [1] and IR-IR-UV hole-burning [2, 3] techniques) it allows for accurate validation of calculated geometries, while together with high-resolution mass spectrometry (2D UV-MS [4]) it allows for an identification of isoforms of biomolecules. These photofragmentation techniques are applicable, however, only to those peptides that contain at least one aromatic residue.