ASSEMBLY STUDIES OF ACETOBACTERIUM WOODII F1FO-ATP SYNTHASE AND ANALYZING CONFORMATIONAL CHANGES OF TMRABBY NESI

摘要

The F1FO ATP synthase of Acetobacterium woodii and the heterodimeric ABC transport complex TmrAB from Thermus thermophilus are responsible for ATP synthesis/hydrolysis and ATP-dependent membrane transport mechanism, respectively. We are interested in the assembly of subunits and investigate requirements for the different assembly steps. For TmrAB we apply ion mobility measurements to analyze conformational changes in dependency of different ligands.