COUPLING GAS-PHASE FRACTIONATION AND PROTON-TRANSFER REACTION FOR IMPROVED TOP-DOWN PROTEOMICS

摘要

The major limitation in top-down proteomics is its reduced throughput. Here, we implement a series of gasphase fractionation strategies on a Thermo Scientific Orbitrap Fusion Lumos Tribrid mass spectrometer to improve the number of detectable proteoforms. Specifically, we applied proton-transfer reaction (PTR) to both deepen the proteome coverage in the 0-30 kDa mass range and to facilitate the analysis of large proteoforms up to ～60 kDa.