Analysis of vitamins D metabolites by isotope-dilution liquid chromatography - tandem mass spectrometry using enzyme-assisted derivatisation

摘要

In addition to being accurate and robust in quantifying the serum level of 25-OHD3, diagnostic MS3 fragment ions of m/z 189 and 205 aid the identification of vitamins D metabolites. Based on the obtained results the presented method can reliably quantify not only 25-OHD3 but vitamins D metabolites in human serum.