High throughput detection of directed evolution driven biotransformation reactions with liquid surface extraction analysis mass spectrometry

摘要

The use of biocatalysts, both enzymes and also engineered whole cells, for producing the target chemicals such as pharmaceutical molecules and intermediates for chemical industrials presents significant advantages in terms of enhanced reaction selectivity, low cost and environmental friendly. The advent of recombinant DNA technology in the late 1970s and the development of directed evolution beginning in the 1990s, can generate popularized candidate enzyme mutants and may extend the narrow substrate scope for industrials, which need a quick, robust and specific approach for screening out the meaningful enzyme and substrate species for the further biotransformation improvement.