首页> 外文会议>ASMS Conference on Mass Spectrometry and Allied Topics >Probing the Stability of Noncovalent Interactions Responsible for Covalently Linked Diubiquitin Ion Structure Using nESI-TWIMS-MS and Collision Induced Unfolding
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Probing the Stability of Noncovalent Interactions Responsible for Covalently Linked Diubiquitin Ion Structure Using nESI-TWIMS-MS and Collision Induced Unfolding

机译:使用NESI-TWIMS-MS和碰撞展开探测负责共价连接的二聚素离子结构的非共价相互作用的稳定性

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CIU provides distinct fingerprints for each of the linkage types of covalent diubiquitin and noncovalent diubiquitin supply ingisight into the relative stabilities of each. Furthermore, it can be concluded that K63 adopts an unquestionably closed conformation at low collision energy and low charge state; that the charge state of the ion has a dramatic effect on the stability of the dimer; and that the addition of methanol induces the collapse of diubiquitin, contrary to the increase in CCS observed for the monomer. Furthermore, comparison of CIU fingerprints for the noncovalent dimer, the hydrophobic interface stabilized K48 dimer, and electrostatically stabilized K63 dimer ions supports previous assertions that the noncovalent dimer is stabilized by hydrophobic interaction.
机译:CIU为共价二聚蛋白和非共价二聚蛋白的每个连杆类型提供了不同的指纹,供应到各自的相对稳定性。 此外,可以得出结论,K63采用低碰撞能量和低电荷状态的无可疑问地闭合构象; 电离离子的充电状态对二聚体的稳定性具有显着影响; 并且添加甲醇的添加诱导二聚蛋白的塌陷,这与为单体观察到的CCS的增加相反。 此外,对于非共价二聚体的CIU指纹的比较,疏水界面稳定的K48二聚体和静电稳定的K63二聚体离子支持上述非共价二聚体通过疏水相互作用稳定的断言。

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