Development and validation of a sensitive LC-MS/MS method for analysis of morphine and its glucuronide metabolites in pediatric plasma and urine

摘要

1. Our LC-MS/MS method with 96-well solid-phase extraction provides an efficient quantification of morphine, M3G and M6G in a small volume (50 μL) of plasma and urine with acceptable accuracy, precision, recovery and LLOQ. 2. This method is currently being successfully utilized for pharmacologic studies of morphine, M3G and M6G in critically ill children to optimize dosing regimens.