Automated N-Glycan Sample Preparation with an Instant Glycan Labeling Dye for Mass Spectrometry

摘要

The characterization of glycans is an essential part of the biotherapeutic development process. Analysis typically involves the labeling of released glycans with a tag to allow for fluorescence detection; a process that often requires numerous hours or days to complete. In addition to fluorescence detection, mass spectrometry is also often utilized. Presented here is the use of an automated N-glycan sample preparation platform with a glycosylamine-reactive dye (InstantPCTM) that provides increased fluorescence and MS sensitivity. Automated N-glycan sample preparation provides reproducible data between replicates and reduced operator involvement.