Rapid analysis of steroid metabolites using field asymmetric waveform ion mobility spectrometry combined with liquid chromatography and mass spectrometry

摘要

FAIMS-MS analysis of seven steroid metabolites shows that all of steroid isobars can be separated using FAIMS apart from DHEAS/TS. Three dimensional analysis using mass extracted ion chromatograms at selected FAIMS CF values (FAIMS) aids the identification and quantification of seven steroid metabolites relevant to anti-doping analysis in sports. Combining FAIMS with chromatography and MS allows for the analysis time to be kept short: 8 mins per run. Steroid glucuronides and sulfates are detected in unspiked human urine using the LC-FAIMS-MS method developed. Further work includes the combination of FAIMS with tandem MS for increased sensitivity to improve LODs and LOQs in the analysis of human urine for anti-doping analysis.