Integration of metabolomic and proteomic profiles with physiological measures to understand the complex mechanisms of sodium fluoroacetate toxicity

摘要

SMFA exposure affected mitochondrial respiration, including basal respiration, ATP production, proton leak, spare respiratory capacity, and coupling efficiency in a dose-dependent manner. The LC/MS analysis quantitated approximately 100 significantly regulated proteins in the N2A cells. The protein and metabolite groups that were identified included those associated with energy metabolism, redox signaling, protein synthesis, and ATP/ADP transport. The quantitative profiling of specific protein sequences in combination with physiological testing provides further understanding of the mechanisms of SMFA toxicity and may aid in identification of new post-exposure treatment targets.