Optimization of LC-ESI (+)-MS-TOF analysis of tropane, harmala and pyrrolizidine alkaloids isolated by MSPD from tropical plants grown in Colombia

摘要

Using experimental design and reference materials (standards), we optimized capillary and fragmentor voltages for LC-ESI-MS analysis. For all alkaloids studied, we observed the higher and more reproducible molecular protonated (MH~+) ion currents at 1000 V-capillary voltage and 150 V or 200 V (monocrotaline) fragmentor voltages. Under these ESI operation parameters, it was possible to detect these alkaloids in a very small plant sample (< 0.1 g), subjected to matrix solid-phase dispersion(MSPD).