Quantitative Profiling of Specialized Metabolites from Transgenic Lines of Camptotheca acuminata Liquid Chromatography, Multiplexed Collision-Induced Dissociation and Time-of-Flight Mass Spectrometry

摘要

Metabolic phenotypes in non-transformed and silenced Camptotheca acuminata were assessed by profiling and quantifying the metabolites in root, stem, shoot apex, young leaf and old leaf tissues. Both cyclase-silenced and tryptophan decarboxylase-silenced lines showed decreased levels of key terpenoid intermediates. Isomers are observed for terpenoid intermediates that have not been reported before in Camptotheca acuminata tissues.