Differential Mobility Spectrometry-Hydrogen Deuterium Exchange (DMS-HDX) as a Probe of Liquid-phase Protein Conformation

摘要

Differential Mobility Spectrometry coupled to gas phase Hydrogen Deuterium Exchange (DMS-HDX) was employed to study liquid-phase protein conformations. With optimised modifier gas, proteins with distinct liquid phase structures exhibited unique deuterium uptake profile as function of compensation voltages (CV).