Determination of Endogenous Cortisol in Human Plasma Using LC-MS/MS Techniques with Combined Calibration Curve and Standard Addition Methods

摘要

The standards were prepared in diluted plasma (1 to 1 ratio) that could be used to mimic and track the behavior of authentic plasma matrix successfully. This bioanalytical method showed acceptable accuracy, precision, selectivity, reproducibility, and sufficient stabilities. The validated bioanalytical method and the established methodology by KCAS have been demonstrated to be simple and reliable in quantitation of endogenous compound in clinical samples using authentic analyte and matrix in preparation of QC samples.