Protein fractionation by subcellular location to enhance proteomic coverage of cultured cells

摘要

Separation of proteins by subcelluar localization reduces sample complexity and increases protein identifications while maintaining biological context. Reproducible fractionation at the protein and peptide level enabled deeper proteomic coverage and more precise assignment of protein localization. Protein expression ratios from TMT ion reporters correlated well with label-free and Western blot analysis.