Identification of Selenocysteine Sites in Selenoproteins using HPLC and Low Energy CID

摘要

We have successfully demonstrated that employing tryptic digestion coupled with HPLC/MS and HPLC/MS/MS using low energy collision is an effective and efficient analytical methodology to locate site of selenocysteine in a selenoprotein. This methodology can definitely be extended to analyze selenoproteins with multiple selenocysteine sites.