Evaluating the Addition of Supercharging Reagents During CESI-MS for Identification and Characterization of Biopharmaceuticals

摘要

Previously, we reported on the integration of capillary electrophoresis (CE) with electrospray ionization (ESI) into one dynamic process which we have termed CESI. The integration of CE and ESI into CESI is achieved by making the end of the separation of capillary porous to ion flow - this is achieved through an HF etching process. This tip is then inserted into a metal needle and filled with a static conductive liquid to establish an electrical connection. Analytes migrate according to their electrophoretic mobility inside the capillary and when it reaches the porous sprayer tip, it is electro-sprayed into MS. The ultra-low flow of the process, yields very high ionization efficiency and serves to reduce ion suppression.